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Ozone Bacterial and 
Mold Killing Studies

Many people want to see the proof that ozone can actually kill bacteria and mold.
These studies that have proven ozone does indeed kill these pathogens in air and water.
Click Here, or scroll below to view the test results proving ozone kills bacteria, mold, and viruses.

Air-Zone® is currently in the process of obtaining independent test results to provide proof our machines are capable of killing mold and bacteria.

While any company can commission a local lab to perform a test for a few hundred dollars, the question is, are the test results truly credible?

One must make sure that the test results given by various companies have meaning in the real world.

To provide test results that have real meaning Air-Zone® intends to provide results from sources that are independent, and completely credible, unlike many of our competitors.

What are the most credible types of scientific tests?
Which test results can truly be trusted?

The tests that have the most credibility are those performed by Universities, the various departments of the US Government,  The Los Alamos National Laboratory, and the non for profit International Ozone Association.

There is one other testing agency that can be assured to have honest, 100% accurate results. 

Those are tests performed by the worlds leading testing company,
the well respected Société Générale de Surveillance otherwise known as SGS.

SGS is the worlds leading verification, testing, and certification company. 
They are the largest testing company in the world, with offices in over 100 countries. 

Their test results are never called into question, because they have the reputation of performing the most honest, accurate, scientific tests in the world.

They will never skew test results to fit a manufacturers agenda, because their reputation is at stake with every test they perform.   Click Here to investigate them for yourself.

Air-Zone® has chosen SGS to test our products so that there can be no possible doubt as to their unparalleled accuracy. The tests we have are available upon request. They are numbered, signed, and stamped with the official SGS seal.

You won't see many other manufacturers using test results from SGS, because their products cannot stand the scrutiny of such accurate tests.

We test our own machines for ppm ozone outputs in real world situations, so that the reader will see that Air-Zone® ozone generators can reach the needed levels of ozone concentration needed to kill bacteria and mold. 

Please understand that while certain manufactures show that their ozone generators can produce ozone levels to kill mold, that that is a test measurement of a ppm level of what their machine can output into a petrie dish, NOT the ozone level that must exist in the ENTIRE room.

While some of our tests show what can be killed in petrie dishes also, understand that our machines can also FILL the ENTIRE room with this level of ozone needed to kill the mold and bacteria over a period of time. This is something no low level ozone generator can do.

An ozone generator that can only produce 20 mg.hr. cannot possibly fill the entire room with a high enough level of ozone since that is all it can produce at it's output vents.
Ozone generators that can ONLY produce a "safe" level of ozone in a room are unable in killing mold in an entire room.

The tests of our machines will show that our units will be able to FILL the ENTIRE room with the levels of ozone needed to kill mold and bacteria.

Please always keep in mind that we produce ozone generators than can produce low levels of ozone for air purification, and that some of our models that can perform High Ozone Shock Treatments that are a MUST to kill mold and bacteria blasted into, of course, UNOCCUPIED rooms.

There is a HUGE difference between these two totally different uses of ozone.
Low levels of ozone, as produced by ozone air purifiers, or ionizers will NEVER be able to remediate mold in an entire room. ONLY the use of unoccupied High Ozone Shock Treatments, that our machines can produce will sterilize rooms of mold and bacteria.

Many manufacturers of low level ozone machines claim their units only produce "safe" levels of ozone and misinform the public that these same machines will kill all the mold in their home. This is simply not true.

Keep in mind that while UV Ultraviolet in duct air purifiers are now popular, their ozone output is very, very low.  No matter what bogus studies the people try and show you understand that UV air purifiers cannot kill mold.

UV Ultraviolet lamps are also ineffective in killing mold and bacteria.
This is a direct quote from General Electric, the manufacturer, concerning their germicidal UV lamps.  "Once mold has already formed, ultraviolet energy will NOT eliminate it."

Click Here to read more about the problems with ultraviolet air purifiers.

60% of all allergies are caused by mold (World Health Organization) 
Mold grows in virtually every air conditioner (EPA) 
Ozone is very effective at killing mold (International Ozone Association) 

Tests have shown that common household bacteria, mold, mildew, and fungus are greatly reduced by the addition of as little as 50 ppb (parts per billion) of ozone in typical household environments.

Pathogens such as, but not limited to, E-Coli, Salmonella Choleraesuis, Staphylococcus Aureua, Candida Albicans, and Aspergillus Niger have been shown to have drastic reductions in population when exposed to ozone, in tests performed by independent laboratories.



Ozone Tests

Results of E coli exposed to airborne ozone as performed by
Penn State University.

SGS Bacterial Test Results of the Air-Zone XT-24000 Ozone Generator

SGS Fungi Test Measurements of the XT-6000 Ozone Generator

Effectiveness of Ozone conducted at the Academy of Medical Sciences in Russia

Additional Ozone / Bacterial / Spore Tests

Off Site Ozone Killing Bacteria / Mold Studies Links

SGS Test Measurements of the Air-Zone XT-301 Ozone Generator

Results of E coli exposed to airborne ozone
Penn State University March 24, 2000
Exposure of E. coli to low levels of airborne ozone
(0.1-0.5 ppm). 
Average ozone level 0.28 ppm. 
 Temperature 75 degrees, 22% relative humidity, 

Test performed by
Penn State Biology Dept.;
W. Kowalski, Brad Striebig, 
T. Whittam, W. Bahnfleth. 

 

High Ozone Shock Treatments Kill Viri and Bacteria!
CFU = Colony Forming Units 
E coli not exposed to ozone
High Ozone Shock Treatments Kill Viri and Bacteria!
 Exposed to ozone 1 hour
High Ozone Shock Treatments Kill Viri and Bacteria!
 Exposed to ozone 2 hours
High Ozone Shock Treatments Kill Viri and Bacteria!
After 3 hours there were no E coli survivors remaining in the petrie dish
"Experiments performed by the authors have found that high bacteria death rates can be achieved with airborne ozone. In these experiments, Escherichia coli and Staphylococcus aureus (MRSA) cultures on plates were inserted in a test chamber through which ozone was pumped. The degree of bacterial disinfection and exposure time compared well with the best results achieved in water by other investigators. 
The ozonation of bacteria in air should produce even higher death rates of the bacteria because of more uniform exposure of the cell surface to ozone. Ongoing research at Penn State has found airborne concentrations of ozone highly effective at disinfecting surfaces. The levels of ozone capable of producing rapid sterilization appear low enough that natural decay, may be sufficient to render the sterilized air breathable without recourse to ozone filtration. The results presented here demonstrate that high degrees of bacterial sterilization can be achieved with airborne ozone."

This is a test of our older model XT-24000 ozone generator performed by SGS proving that this machine can produce an ozone level high enough to kill 99.5% Bacillus subtilis in just 5 minutes.
This report is published with the permission of SGS and may not be reproduced in any form whatsoever. Copyright Air-Zone Inc. 2004

A copy of this test, numbered and signed by SGS,
stamped with the SGS official seal, is available upon request.

The XT-28000 is can achieve an ozone level of 3 parts per million in a 1700 cu.ft.. room within one minute. 

After 30 minutes, at a distance of 12 feet the ozone level reached 16 parts per million.

This is the type of power required to kill mold and bacteria.

These are the test results of the older, less powerful, model XT-24000.


This is a recent test of our XT-6000 ozone generator performed by SGS proving that this machine can produce an ozone level high enough to kill fungi in 30 minutes.
This report is published with the permission of SGS and may not be reproduced in any form whatsoever. Copyright Air-Zone Inc. 2004
A copy of this test, numbered and signed by SGS,
stamped with the SGS official seal, is available upon request.

Air-Zone Test Measurements of the XT-6000 Ozone Generator


Air-Zone XT-6000

Ambient room temperature: 80.3 F
Room size: 17 x 12 x 8.3 foot ( 1693 cubic feet ), no ventilation
Ozone Concentration: 22 ppm, Ozone Air Flow Rate: 82 CFM
Ozone Output Per Hour: 6500 mg/hr. 
 

Test Time
5 minutes
10 minutes
30 minutes
45 minutes
Ozone Concentration
0.3 ppm
0.55 ppm
1.0 ppm
0.9 ppm
Test conducted with the measuring instrument at a 5 foot distance from the ozone generator.


Test Time
10 minutes
60 minutes
Ozone Concentration
0.3 ppm
0.9 ppm
Test conducted with the measuring instrument at a 12 foot distance from the ozone generator.
Test of Nitrogen Oxides, NO+NO2:
The ozone measuring sensor was placed directly in front of the ozone generator.
Zero Nitrogen Oxide levels could be detected by the measuring device.

Test Conclusions:

The ozone concentration is dependent upon the location, and running time of the ozone generator. The 12 foot distance used in the second study is equivalent to placing the unit anywhere in the designated area. Centered in the middle of the room, the unit was never more than 8.5 feet from the furthest point in the room.

The ozone generator was found to easily obtain an ozone concentration level of at least 0.3 ppm within 10 minutes of operation, no matter where in the room the unit was placed, or where the ozone concentration was measured.

It has been previously proven in University of Pennsylvania studies that E coli bacteria will be killed at average 0.28 ppm ozone concentration within two hours.

In our opinion it can then be reasonably concluded that the Air-Zone XT-6000 ozone generator will kill 99% of all E coli bacteria, in a 1700 cubic foot room within a time period of 60-90 minutes.

We thus make these conclusions since higher ozone concentrations than needed to kill 
E coli bacteria can be obtained in a shorter period of time with the Air-Zone XT-6000.

Other recent studies have also proven that ozone concentrations within 0.10 ppm and 0.40 ppm
will kill most any bacteria or viruses in a relatively short period of time.

It is then reasonably safe to conclude that most other pathogens will also be destroyed by the Air-Zone XT-6000 within a short period of time.

It is important to note that  these high levels of ozone needed to kill bacteria, can only be used in rooms unoccupied of people, plants, and animals.

Test conducted October 2001 

Effectiveness of Ozone
In studies conducted at the Academy of Medical Sciences in Russia, Dr. Gubernskii and Dr. Dmitriev found that 0.005 ppm (parts per million) to 0.02 ppm of ozone added to normal indoor air (0 ppm) increased animals' resistance to the cold, to infection, to toxic substances, and to oxygen deprivation. A general increase in the immune "biological potential" and the vital capacity of the lungs was reported.

Dr. Gubernskii and Dr. Dmitriev also performed tests using less than .01 ppm of ozone in an air-conditioned office building, which revealed that "the levels of oxygen in the blood increase relatively quickly and remain at a high level for the duration of the experiment." They also stated that: "Atmospheric ozone has a positive effect on animals and people. It is important to note its positive effect on the breathing system, blood composition, arterial pressure, immune system, general feeling of well being, and mental and physical work capability. 

Additional Ozone Bacterial Tests

Here are some other tests that are too lengthy to publish on this web site. They consist of scanned jpegs that can be downloaded here in the zip file format. 
Just click on the file name and download files.  These files can be opened with Winzip or any unzipping program. They have been scanned for viruses. 

The Deactivation of Dirty Spores (including Anthrax) by Ozone
Conducted by The Los Alamos National Laboratory
file size 1.73 MB

Bacteriacidal Effects of High Airborne Ozone Concentrations
on Escherichia coli Staphylococcus aureus
Conducted by Penn State University

Quote: "The results presented here demonstrate that high degrees of bacterial sterilization can be achieved with airborne ozone". . . . "The results suggest that efficient sterilization of airstreams could be accomplished in a matter of seconds by the use of ozone."

This can be downloaded this as a zip file of scanned jpegs HERE.
file size 1.84 MB

Click Here if you need Winzip to unzip these files. 



Off Site Ozone Test Links
 

Purdue University finds that ozone kills mold in grain.

Impact summary: Insects eat $12 million worth of stored grain in Indiana each year, and pesticides have been the tool that grain handlers use to stop them.   Purdue researchers, looking for ways to reduce pesticide use, found a potential alternative in ozone fumigation.   In initial tests, ozone fumigation killed more than 90 percent of major insect pests and cut Aspergillus fungus populations by more than half.   It leaves no residue in grain, does not escape into the environment, and appears to be economically competitive. 
Title: Ozone fumigation could replace pesticides in stored grain.

The United States Food and Drug Administration approves the use of ozone
for use in killing bacteria in food.

The United States Food and Drug Administration approves the use of ozone for use in food.
They state that ozone may be safely used in the treatment, storage, and processing of foods as a antimicrobial agent.

The Food and Drug Administration has approved the use of ozone in gaseous and aqueous phases as an antimicrobial agent on food, including meat and poultry. Responding to a petition from the Agriculture and Food Technology Alliance of the Electric Power Research Institute, Palo Alto, Calif., FDA published a final rule that permits use of ozone as a food additive (66 Fed. Reg. 33,829). 

Purdue University finds that ozone kills, mold, mold mycotoxins and insects.

Purdue University, in follow up studies, determine that ozone safely kills mold, mold mycotoxins, and insects in grain stores.  They state that while ozone kills the deadly mold fungi of Fusarium and Aspergillus, that ozone is safer than chemicals. 

Linda Mason, Purdue entomology associate professor and co-author of the study  states:
"The chemicals currently used can kill everything in and around the grain bin, including people. 
With ozone, we're not generating ozone at deadly concentrations, and we have better control over it when it's present."

Dirk Maier, a Purdue agricultural and biological engineering professor, studied how to make the ozone flow efficiently and effectively through grain storage bins. 

Charles Woloshuk, a botany and plant pathology professor, studied ozone effects on molds and mycotoxins. 

The scientists began their study after a company that uses ozone air purification systems in hospitals noticed that air vents were cockroach free. 

Absence of cockroaches in a large building is unusual, so the researchers tested various ozone doses on different insects and found the gas was fatal to bugs.

The International Ozone Association states that ozonated water helps fight MRSA (staph infections)

Ozone Kills MRSA "Super Bugs"By Paul Overbeck, Nov 1, 2007

SCOTTSDALE, AZ - The International Ozone Association (IOA), based here, says that sanitizing towels, linens and surfaces with ozonated water has been shown to be "extremely effective" in the reduction of the staph infection "super bug" MRSA, a methicillin-resistant form of Staphylococcus aureus which has been spreading across the United States, according to a recent IOA press release. 

The Centers for Disease Control and Prevention (CDC) recently reported that in 2005, MRSA, which can start as a serious skin infection, was responsible for more than 94,000 infections and about 19,000 deaths in the United States.

The IOA reports that washing with ozonated water can reduce MRSA and its conventional S. aureus cousin by as much as 99.999999 percent. The CDC has said that good handwashing practices and other forms of hygiene also help prevent MRSA infections, which have been most commonly contracted in health care facilities but have been shown in recent media reports to have occurred in schools.

A paper released last year by the International Ozone Association,"Ozone in the Laundry Industry - Practical Experiences in the United Kingdom," said that tests by Microsearch Laboratories confirmed that low-temperature ozone washing can effectively inactivated organisms including Clostridium difficile, E. coli, MRSA, Pseudomonas aeruginosa, Candida albicans, Streptococcus faecalis, Aspergillus niger, Clostridium perfringens, Campylobacter jejuni, and Aeromonas, Actinobacter and Lactobacilli.

The International Ozone Association is a non profit organization so its studies
cannot be published on private websites which is why we do not post it here.

These are news stories about the study.
Ozone Kills MRSA "Super Bugs"

MRSA 'super bug' not ozone resistant


The Sports-O-Zone is a patented machine that destroys harmful bacterial growth that produces odors in sports protective gear and garments.

The Sports-O-Zone machine is manufactured exclusively with Air-Zone® brand
Ozone Generators!

Click Here to see the Sports-O-Zone news report.
Click Here to see the
Sports-O-Zone news report.


SGS Test results of the Air-Zone XT-301
Air and Water Ozone Purifier
This test measures the ability of the Air-Zone XT-301 Ozone Purifier to kill different forms of bacteria in water.
Quote from SGS: "The ozone purifier was selected at its level 4 ozone output. Ozone was pumped from the machine by method of a tube immersed into solution of bacterially contaminated water for thirty minutes.  The test method of the bacteria count was according to the United States FDA Bacteriological Analytical Manual."

Test Results

Tested Bacteria
Initial Bacterial Count
CFU / ml
Remnant Bacterial Count
CFU / ml
Disinfect Efficiency
%
Coliform
1.8 X 10 to the 6th
<10
99.9%
Salmonella
6.0 X 10 to the 3rd
<10
99.9%
Staphylococcus
Aureus
7.0 X 10 to the 5th
<10
99.9%
Pseudomonas Aeruginosa
5.7 X 10 to the 4th
<10
99.9%
Fecal Streptococci
8.2 X 10 to the 4th
<10
99.9%
Aspergillus Niger
(mold)
0 Hours
2.0 X 10 to the 4th
CFU / ml
0.5 Hours
4.0 X 10 to the 1st
CFU / ml
3 Hours
<1
A copy of this test, numbered and signed by SGS,
stamped with their official seal, is available upon request.


These test results  may not be reproduced in any manner or form without prior written permission from Air-Zone Inc. and SGS International.  These test results are copyrighted by Air-Zone Inc. 2003

More ozone killing bacteria, virus, and mold studies will be available soon.

Air-Zone® Ozone Generators are the most effective tool for removing mold, smoke, pet, and many other stubborn odors.

Air-Zone® Ozone Generators safely 
KILL MOLD including Toxic Black Mold.

Air-Zone® Ozone Machines are thousands of times more powerful than simple ionizers, which cannot possibly kill substantial amounts of mold and bacteria. 

Click Here to play our Mold Killing Movie!

Here are some various links about the effectivness of ozone.

Effect of ozone on concentrations of lysozyme in phagocytizing alveolar macrophages

Ozone has amazing powers

Ozone in Medicine: Overview and Future Directions

In today's times, when everyone is concerned with many types of
infectious bacteria, it is a good idea to sterilize rooms with
High Ozone Shock Treatments.

Killing germs, bacteria, and viruses with ozone may be one of the best ways to protect your family 
from the complex array of diseases we face today.


High Ozone Shock Treatments
are an excellent way to kill bacteria & germs

Air-Zone ozone generators help kill mold and bacteria
by attacking them at the source.

As shown in the diagram above, simple Oxygen molecules known as O2, can be turned into Ozone, O3, by the introduction of the high voltage electrical charge given by an Air-Zone Ozone Generator.

Every ozone generator owner should try High Ozone Shock Treatments for sanitizing areas. 
Many times the shock treatment will be enough to freshen the room and the ozone need not be on when the room is occupied. Some individuals are sensitive to even low levels of ozone and High Ozone Shock Treatments are the best method for them to use ozone for great benefit when they are not in the room. the effects of ozone continue to last even after the generator is turned off.

High Ozone Shock Treatments MUST be used to remove mold odors, mold spores, viruses, bacteria, and severe odors such as smoke, or pet odors.

Recommended ozone generators for
High Ozone Shock Treatments

XT-2000
XT-4000
XT-6000
Air-Zone XT-14000
Professional High Powered Ozone Generator
Patented Ozone Elements
Produces Zero Nitrogen Oxides
21st Century High Tech Design
BUILT IN 24 HOUR TIMER
Features astounding ozone outputs of
14000 mg/hr!

Extra Thick Rust & Oxidation Proof 
Stainless Steel Case & Chassis

CLICK HERE
to learn more about the amazing XT-14000!

Amazing Power, Professional Performance, Unbelievable Price!
Air-Zone XT-28000
Professional High Powered Ozone Generator
Patented Xetin II Ozone
Produces Zero Nitrogen Oxides
21st Century High Tech Design
BUILT IN TIMER
Features astounding ozone outputs of
9300, 18600, or 28000 mg/hr!

Extra Thick Rust & Oxidation Proof 
Stainless Steel Case & Chassis

CLICK HERE
to learn more about the amazing XT-28000!

Extremely High Ozone Output!  Perfect For Professional Applications!  Zero Nitrogen Oxides!
Please call us for ozone generator sizing information.
We carry a full line of OZONE GENERATORS
Click Here to see our entire line
Perfect for a bedroom or office
 Please call us so we can best advise you
which is the right model for your situation. 

 
Air-Zone Ozone Generators is proudly located in the U.S.A.!!!
Air-Zone Inc.
1980 Northgate Commerce Parkway, Suite 6C
Suffolk, VA  23435

Call our 1-800 TOLL FREE number
1-800-772-5142 

Outside the 48 Continental US States please call
757-465-1121
Open Evenings & Saturdays!

Call to reach our staff of qualified ozone technicians.
If all of our phone lines are busy please leave a message and we will call you back as soon as possible.

Main Email Contact:
AirZoneAir@aol.com


Thinking of buying a different brand of air purifier / ozone generator?
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